Objective:To establish a content determination method of Astragaloside in Huangqi Shengluo Fukang Pill by HPLC -ELSD. Methods:The chromatographic Column Diamonsic C18(4.6mm ×250mm,5μm)was set.The mobile phase consisted of acetonitrile and water (35:65), and the flow rate was 1.0mL· min-1.The column temperature was 40℃,and the content was determined with evapo-rate light scattering detector ( ELSD) .The temperature of spray chamber was 80℃and the temperature of evaporator was 100℃.The gas flow rate of parameter of ELSD was 1.5mL· min-1 .Results: The linear range of Astragaloside was within 1.62 to 8.10μg ( r=0. 9999).The average recovery rate was 99.08%(RSD=1.63%.Conclusion: The established method is convenient and accurate and highly specific , and it can be used for the quality control of Huangqi Shengluo Fukang Pill .%目的:采用HPLC-ELSD法建立黄芪生络复康丸中黄芪甲苷的含量测定方法。方法:色谱柱为Diamonsic C18(4.6 mm ×250 mm,5μm),流动相为乙腈-水(35∶65)为流动相,流速为1.0 mL· min-1,柱温为40℃。 ELSD检测器,雾化器温度80℃,蒸发器温度100℃,载气流速1.5 mL· min-1。结果:黄芪甲苷进样量在1.62~8.10μg时线性关系良好,r=0.9999,平均加样回收率为99.08%( RSD=1.63%)。结论:本方法简便、准确、专属性强,可用于本品的质量控制。
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