慢性束缚应激抑郁症大鼠海马Belin-1、LC3的表达变化及逍遥散的调节作用

摘要

Objective:To observe the effects of Xiaoyaosan Decoction on the expression of Belin-1 and LC3 in hippocampus of rats with depression induced by chronic restraint stress.Methods:A total of 20 Wistar rats were randomly divided into 4 groups:the control groupthe model groupthe Xiaoyaosan (XYS) group and the fluoxetine group.Depression model was established by 21-day chronic restraint stressand the expression of Belin-1 and LC3 in CA3 of rats′ hippocampus was detected by immunohistochemistry.Results:There is significant differences between the control group and other groups (P<0.05).Xiaoyaosan Decoction could down-regulated the expression of Belin-1 and LC3 (P<0.05).Conclusion:The expression of Belin-1 and LC3 in hippocampus of rats with depression induced by chronic restraint stress indicated that autophagy may be part of the mechanism of depression.The effect of Xiaoyaosan Decoction on depression could be related to the regulation of Belin-1 and LC3.%目的:观察慢性束缚应激抑郁症大鼠模型中海马自噬标志蛋白Belin-1及LC3的表达变化情况,探讨逍遥散对其的调节作用.方法:20只Wistar大鼠随机分为4组(n=5):正常组、模型组、逍遥散组、氟西汀组.采用21 d慢性束缚应激方法建立抑郁症大鼠模型,利用免疫组织化学方法检测大鼠海马CA3区Belin-1、LC3表达情况.结果:Belin-1、LC3在各组中较正常组均有较明显的表达(P<0.05).与模型组相比,逍遥散能明显下调Belin-1、LC3的表达(P<0.05).结论:慢性束缚应激抑郁症大鼠海马自噬相关蛋白Belin-1、LC3表达有变化,提示自噬可能是抑郁症发病机制之一,且逍遥散治疗抑郁症的机制可能与调节自噬标志蛋白Belin-1、LC3有关.