人参皂苷20(s)-Rg3对llc-pk1细胞中顺铂所致肾毒性的保护作用

摘要

Objective:To discuss protective effects of ginsenoside 20 ( s)-Rg3 on renal toxicity induced by cisplatin in llc-pk1 cells.Methods:This article studied the protective effect of fermentation of black ginseng cells (FBG) and its active component ginsenoside 20 (S)-Rg3 on porcine kidney (LLC-PK1) cells in cisplatin (chemotherapy)-induced injury based on kidney protec-tion test.Results:The cisplatin induced cell viability decreased , and then FBG was used to extract and ginsenoside 20 (S)-Rg3 was restored by FBG.After the dose dependent or extract ginsenoside 20 (S) after-Rg3 treatment may reduce cisplatin induced in-creased phosphorylation of c-Jun and N-terminal kinase (JNK), p53 and caspase cleavage the increase of-3 protein.By using FBG and 20 ginsenosides (S) together with-Rg3, the percentage of cisplatin induced apoptosis in LLC-PK1 cells leaded to increased significantly.Conclusion:FBG and its major ginsenoside 20(S)-Rg3, ameliorated cisplatin-induced nephrotoxicity in LLC-PK1 cells by blocking the JNKep-53-ecaspase-3 signaling cascade.%目的:探讨人参皂苷20(s)-Rg3对llc-pk1细胞中顺铂所致肾毒性的保护作用.方法:通过基于细胞的肾脏保护试验,研究了发酵黑参(FBG)及其活性成分人参皂苷20(S)-Rg3对猪肾(LLC-PK1)细胞中顺铂(化疗药物)诱导的损伤的保护作用.结果:由顺铂诱导的细胞活力降低,再使用FBG提取物和人参皂苷20(S)-Rg3依赖剂量后明显恢复.用FBG提取物或人参皂苷20(S)-Rg3处理后会降低顺铂诱导升高与磷酸化c-Jun N-末端激酶(JNK),p53和裂解的半胱天冬酶-3升高的蛋白.通过用FBG和人参皂苷20(S)-Rg3的共同处理,由于顺铂的诱导导致凋亡LLC-PK1细胞升高的百分比显著降低.结论:人参皂苷20(s)-Rg3可改善LLC-PK1的细胞毒性,人参皂苷20(S)-RG3可能是通过阻断JNK-P53-caspase-3信号级联反应来介导这种作用的重要组成部分.