目的:探讨归芪多糖对免疫低下小鼠迟发型超敏反应(DTH)的影响.方法:将40只昆明种小鼠随机分为正常对照组、模型对照组、归芪多糖高剂量组(100 mg/kg)、归芪多糖低剂量组(50 mg/kg)和阳性药物对照组[黄芪精口服液(1.8 g/kg)],每组8只.除正常对照组外其余各组通过皮下注射环磷酰胺(CY)建立免疫抑制小鼠模型.模型对照组灌服等体积生理盐水,各药物组分别灌服归芪多糖(50 mg/kg和100 mg/kg)和黄芪精口服液(1.8 g/kg),1次/d,连续灌服6天后,除正常对照组外其余各组以二硝基氟苯(DNFB)致敏并加强2天后计算小鼠耳廓肿胀度及脾脏、胸腺指数.结果:100 mg/kg归芪多糖能明显增加免疫抑制小鼠耳廓肿胀度,与阳性对照药物黄芪精口服液相比无显著性差异;且归芪多糖在50～100mg/kg剂量范围内可对抗环磷酰胺所致小鼠脾脏和胸腺的萎缩,增加小鼠免疫脏器指数.结论:归芪多糖可恢复小鼠对外源性过敏原所导致的迟发性超敏反应,且能对抗环磷酰胺引起的脾脏和胸腺的萎缩.%Objective: To explore the effects of angelica and astragalus polysaccharide on delayed type hypersensitivity (DTH) of imm unosuppressive mice. Method: All 40 mice were random ized into normal control group, model control group, high (100 m g/kg) and low (50 m g/kg) dosage groups of angelica and astragalus polysaccharide, and the control group of positive drug (HuangQiJing oral liquid, 1.8 g/kg), eightm iceeach group. Except normal control group, imm unosuppressive mice models were established with hypodermic injection of cyclophospham ide (CY). N ormal control group received the sam e am ountof physio logical saline and other groups were given with angelica and astragalus polysaccharide (100 m g/kg and 50 m g/kg) and HuangQiJing oral liquid (1.8 g/kg), once each day, for six consecutive days, exceptnormal control group, other groups w ere sensitized with dinitrofluorobenzene (DNFB) and enhanced fortwo days to calculate the swollen degree of auricle, the indexes of spleen and thym us. Result: Com pared with HuangQiJing oral liquid, 100m g/kg angelica and astragalus polysaccharide enhanced the swollen degree of auricle and showed no significant difference; angelica and astragalus polysaccharide could resist the atrophy of spleen and thymus in mice induced by CY and enhance the index of imm une organs in mice. Conclusion: Angelica and astragalus polysaccharide could recover delayed type hypersensitivity and resist the atrophy of the spleen and thym us induced by C Y.
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