Development of RNA Polymerase Ⅲ-Driven Reverse Genetics System for the Rescue of a Plant Rhabdovirus

摘要

Dear Editor,De novo generation of negative-stranded RNA viruses(NSVs)requires efficient transcription of integral viral RNAs with precise termini from cloned plasmids.Studies during the past 25 years with animal NSVs have established the bacteriophage T7 RNA polymerase(Pol)-and endogenous Pol I-based transcription systems as the most efficient platforms for recovery of recombinant NSVs(Bridgen 2012).Unfortunately.