三氧可通过减轻小鼠脑小胶质细胞激活预防抑郁症发生

摘要

Objective Explore the impact of ozone on depression model, including the behaviors, the number of microglia cell and inflammation cytokines (IL-1β,IL-6 and TNF-α) in brain hippocampus, prefrontal cortex and amygdaloidal nucleus regions. Methods Forty mice were randomly divided into 4 groups; stress+saline (10), stress+ozone (10), control+saline (10), control + ozone (10). The model mice were set in a special centrifuge tube for 1 hour per day, lasting for a week, ozone by intraperitoneal injection of control + ozone and stress+ozone. Giving ozone 3 days before depression model as a ozone previously intervention. Giving ozone after depression model as a ozone therapeutic intervention. Tail suspension test (TST), forced swimming test (FST), novelty suppressed feeding test (NSFT), open field test (OFT) were subjected to detect the behavior of each group. Using the immunohistochemical technology to detect the number of microglia cell in brain hippocampus, prefrontal cortex and amygdaloidal nucleus regions. Using reverse transcription polymerase chain reaction technology to detect the expression level of inflammatory cytokines IL-1β,IL-6 and TNF-a from hippocampus, prefrontal cortex and amygdala region. Results After ozone previously intervention, ozone could improve depression behaviors in mice, reduce the number of activated microglial cells in the prefrontal cortex and hippocampus, decrease the levels of inflammatory cyto-kines IL-1β, TNF-α. After ozone intervention, ozone could not improve the depression behaviors in mice. There was no significant difference in the number of activated microglial cells in the prefrontal cortex, hippocampus and amygdala between stress+saline group and stress+ozone group (P＞0.05), ozone treatment failed to reduce the levels of IL-1β and TNF-a in the prefrontal cortex and hippocampus. Conclusion Ozone intraperitoneal injection has some effects on preventing the formation of depression, but there is no therapeutic effects on depression.%目的 探究三氧对抑郁症模型小鼠行为学以及脑内海马、额叶和杏仁核小胶质细胞数量与炎症因子水平的影响.方法 将40只小鼠随机分为4组,模型组、模型加三氧组、对照组和对照加三氧组,每组10只.对模型组和模型加三氧组小鼠进行连续7 d,每天1 h的束缚应激制备抑郁症小鼠模型,其中模型加三氧组和对照加三氧组采用三氧水(80 μg/mL)腹腔注射.造模前3天给予三氧作为三氧预防性干预,造模成功后给予三氧作为三氧治疗性干预.利用悬尾实验、强迫游泳实验、新环境抑制进食实验和开放旷场实验检测各组小鼠行为学变化.小胶质细胞标记物IBA1免疫组化染色检测海马、额叶和杏仁核小胶质细胞数量的改变.逆转录聚合酶链式反应技术检测海马、额叶和杏仁核炎症因子白介素(inerleUkin,IL)-1β、IL-6和肿瘤坏死因子(tumer necrosis faCtor-α,TNF-α)的表达水平.结果 三氧预防性干预中:三氧能改善小鼠抑郁行为,减少脑内额叶、海马区激活的小胶质细胞数量,降低炎症因子IL-1β、TNF-α的水平.三氧治疗性干预中:三氧未能改善小鼠抑郁行为,模型加三氧组与模型组额叶、海马、杏仁核区激活的小胶质细胞数量比较差异无统计学意义(P＞0.05),三氧治疗性干预未能降低额叶、海马区炎症因子IL-1β、TNF-α的水平.结论 三氧腹腔注射对预防小鼠抑郁症形成有一定的作用,但无治疗作用.