赤芍801对白细胞介素-6与其受体相互作用影响的研究

摘要

白细胞介素-6的可溶性受体(sIL-6R)在石英晶体微天平基片表面固定的量为297.08ng·cm-2.白细胞介素-6(IL-6)可以特异性结合sIL-6R,结合的量为33.70 ng·cm-2.IL-6的响应具有计量依赖性.根据上述分析,得到kass值为1.27×104(mol/L)-1s-1,kdiss值为1.94×10-4s-1,KD值为1.53 ×10-mol/L.表明赤芍801能够增加IL-6与其受体的结合,从而影响IL-6及其受体的生物学活性.可以推断出IL-6与其受体的结合是赤芍801在体内的另一个作用靶点.%The immobilized amounts of interleukin-6 soluble receptor (sIL-6R) on the surface of quartz crystal microbalance (QCM) substrate are 297.08 ng · cm-2. interleukin-6 (IL-6) can specifically bind to sIL-6R and the bound amounts are 33.70 ng · cm -2. The response for IL-6 is dose-dependent. Based on the analysis, the value for kassis 1.27 × 104 (mol/L) -1 s -1 , the value for kdissis 1.94 × 10 -4 s -1 , the value for KD is 1.53 × 10 -8 mol/L.Paeoniae radix 801 can enhance the binding amounts of IL-6/sIL-6R, which is dose-dependent. IL-6/sIL-6R is another action target of Paeoniae Radix 801 in vivo.