Objective To explore expression of Beclin1, autophagy-related protein 1 (Atg1), mammalian target of ra-pamycin (mTOR) in squamous cell carcinoma (SCC) and adenocarcinoma of lung and to analyse relationship among these three factors. Methods Immunohistochemical stainning was applied to detect expression of Beclin1, Atg1 and mTOR in 82 samples of SCC and adenocarcinoma of lung (lung cancer group) and 17 samples of paraneoplastic normal lung tissues (con-trol group). Correlations of expression of Beclin1, Atg1 and mTOR were analyzed in SCC and adenocarcinoma of lung. Re-sults The expression of Beclin1 was significantly lower in lung cancer group than that in control group(52.44%vs 94.12%, P<0.01). There were significant differences of the differentiation, TNM stages and lymph node metastasis in lung cancer group compared with those in control group. And Beclin1 expression showed a decreasing trend with malignant and invasive of tumors. mTOR expression in lung cancer tissue with lymph nod metastasis is higher than that in the tissue without lymph node metastasis(P<0.01). Atg1 expression in lung cancer tissue with larger mass is less than that in lung cancer tissue with small lumps(P<0.01). Beclin1 Expression was positively correlated with the Atg1 expression in SCC(r=0.609,P<0.01). Beclin1 and Atg1 expressions were both negatively correlated with mTOR expression in SCC(r=-0.617,-0.444,P<0.01). Beclin1 expression was positively correlated with Atg1 expression in adenocarcinoma(r=0.458,P<0.01). Beclin1 and Atg1 expression were both negatively correlated with mTOR expression in adenocarcinoma(r=-0.497,-0.541,P<0.01). Conclu-sion Beclin1, Atg1 and mTOR expression showed a strong correlation in lung cancer, and clinicopathological change of lung SCC and adenocarcinoma may be related to autophagy.%目的：探讨肺鳞癌和腺癌中自噬相关蛋白Beclin1、自噬相关蛋白1（Atg1）、雷帕霉素靶蛋白（mTOR）的表达情况及其相关性分析。方法采用免疫组织化学Envision二步法，检测82例肺癌（鳞癌和腺癌）组织及17例癌旁正常肺组织中Beclin1、Atg1、mTOR的表达情况，并分析其表达的相关性。结果肺癌组织中Beclin1的阳性表达率低于正常组（52.44%vs 94.12%，P＜0.01），在淋巴结转移、TNM分期、不同分化程度情况的肺癌组织中阳性表达率差异有统计学意义，且随肿瘤恶变和侵袭程度的增强呈递减趋势。肺癌组与正常组Atg1阳性表达率比较差异无统计学意义（64.71%vs 46.34%），但在肿块较大肺癌组织中的阳性表达率低于肿块较小者（P<0.01）。肺癌组mTOR阳性表达率高于正常组（53.66%vs 5.88%，P＜0.01），有淋巴结转移的肺癌组织中mTOR阳性表达率高于无淋巴结转移者（P<0.01）。相关性分析显示，鳞癌中Beclin1与Atg1表达呈正相关（r=0.609），Beclin1、Atg1分别与mTOR呈负相关（r分别为-0.617、-0.444）；肺腺癌中，Beclin1与Atg1表达呈正相关（r=0.458），Beclin1、Atg1分别与mTOR呈负相关（r分别为-0.497，-0.541），均P<0.01。结论 Beclin1、Atg1、mTOR在肺鳞癌和腺癌组织中的表达呈现较好的相关性，提示肺鳞癌和腺癌的临床病理学变化与自噬有关。
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