YY1基因在肺癌组织中的表达及其对肺癌细胞功能的影响

摘要

Objective To investigate the expression of transcriptional factor YY1 in lung cancer tissues and discuss its function in cell proliferation, migration and invasion. Methods The mRNA expression of YY1 was determined by real-time quantitative PCR in 235 samples of lung cancer tissues and 62 samples of adjacent tumor tissues, and its relationship with clinical pathological characteristics was analyzed. Non-small cell lung cancer (NSCLC) cell lines were transfected with YY1 over-expression vector and negative control vector using Lipofectamine 3000. The proliferation of cells was measured by MTT in control group and YY1 over-expression group after 24 h, 48 h and 72 h of transfection in A549 and H1299 cells. Wound-healing assay and Transwell assay were performed to investigate the effect of YY1 over-expression on the invasion and migration of lung cancer cells. Results Compared to normal adjacent tissues (5.80 ± 0.58), the expression of YY1 mRNA was significantly lower in lung cancer tissues (5.07 ± 0.58, P < 0.01). There were significant differences in the expression levels of YY1 mRNA between gender, smoking status, histology subtypes or clinical stages (P<0.05). Furthermore, compared to control group, the over-expression of YY1 was found to suppress the proliferation for 48 h and 72 h in H1299 cells (P<0.01), and A549 cell for 72 h (P<0.05). Wound-healing assay and Transwell assay showed that the over-expression of YY1 inhibited migratory and invasive capability of lung cancer cells (P < 0.05). Conclusion The expression level of YY1 mRNA is lower in lung cancer tissue, and YY1 suppresses proliferation, migration and invasion of lung cancer cells.%目的：检测转录因子YY1在肺癌组织中的表达情况，并分析其对肺癌细胞的增殖、迁移及侵袭的影响。方法应用Taqman探针实时荧光定量PCR方法检测235例肺癌组织以及62例配对癌旁组织中YY1 mRNA的表达，并分析其表达与患者临床病理特征的关系。应用Lipofectamine 3000试剂在肺癌细胞系转染YY1过表达质粒及对照质粒。噻唑蓝（MTT）分别检测对照组和YY1过表达组在24、48及72 h时A549和H1299细胞增殖能力的变化。划痕实验和Transwell实验检测YY1过表达对肺癌细胞的迁移和侵袭能力的影响。结果与癌旁组织（5.80±0.58）相比，YY1 mRNA在肺癌组织（5.07±0.58）中表达下调（P<0.01），且YY1 mRNA的表达在不同性别、吸烟状态、病理类型及临床分期中差异有统计学意义（P<0.05）。YY1过表达组H1299细胞增殖能力在48 h和72 h均低于对照组（P<0.01），A549细胞在72 h低于对照组（P<0.05）。划痕实验和Transwell实验结果显示转染过表达YY1质粒后，肺癌细胞的侵袭能力及迁移能力明显下降（P<0.05）。结论 YY1在肺癌组织中低表达，上调YY1的表达可抑制肺癌细胞的增殖、迁移和侵袭。