目的：探讨应用富血小板纤维蛋白（PRF）对牵引成骨区骨形态发生蛋白-6（BMP-6）表达的影响。方法25只大耳白兔随机分5组，分别行双侧下颌骨皮质骨切开术，一侧下颌骨牵引间隙放置PRF膜，作为实验组，对侧作为对照组，分别于稳定期第1、3、7、14、28天处死1组动物，切取牵引间隙处骨痂行HE染色和BMP-6免疫组织化学染色，细胞图像分析仪测量牵引间隙处骨痂BMP-6表达情况。结果下颌牵引延长后牵引间隙均有新骨形成，免疫组化染色显示BMP-6主要定位于成骨细胞的胞浆中。实验组在稳定期第1、3、7天BMP-6表达的阳性细胞率和阳性面积百分比均高于对照组（P＜0.05），稳定期第14、28天BMP-6表达的阳性细胞率和阳性面积百分比均与对照组比较差异无统计学意义。结论 PRF能促进兔下颌骨牵引成骨区新骨的生成，BMP-6可能在牵引成骨过程的早期调控组织细胞应力信号传递，发挥成骨作用。%Objective To investigate the effects of platelet-rich fibrin (PRF) on BMP-6 expression during mandibular distraction osteogenesis. Methods Twenty-five mature rabbits were randomly divided into five groups. Mandibular osteoto⁃mies were performed corticectomy in bilateral mandibles of rabbits. PRF was implanted in the one side of mandibles, which was used as experimental group. Another side of madibular was used as control group. Rabbits were sacrificed and the dis⁃tracted calluses were harvested and processed for HE and BMP-6 immunohistochemistry staining at 1, 3, 7, 14 and 28 days after the end of distraction, respectively. The expressions of BMP-6 in the distracted calluses were analyzed by cell digital imaging software. Results The regenerated bone was found in the distraction gap after mandibular lengthening. Expres⁃sions of BMP-6 were co-localized in cytoplasm of osteoblasts and newly embedded osteocytes. Compared with control group, the positive cell rate and positive area percentage of BMP-6 were significantly higher on the 1st, 3th and 7th day after the distraction in experimental group (P<0.05). There were no significant differences in positive cell rate and positive area per⁃centage of BMP-6 at 14-day and 28-day after the distraction between experimental group and control group. Conclusion PRF can accelerate bone formation of mandibular distraction osteogenesis in rabbits. BMP-6 may play important role at the early stage of mandibular distraction.
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