CE法分离检测对羟基苯丙酮酸互变异构体

摘要

The enol-keto tautomerism of p-hydroxyphenylpyruvic acid was investigated by capillary electrophoresis with amperometric detection.A three electrode system with a carbon microelectrode (i.d.0.3mm) as the working electrode was used.The two tautomers were separated and detected within 15min at 18kV of applied voltage and a 60cm (i.d.25μm) length capillary in 50mmol/L phosphate buffer (pH=6.60) including 3mmol/L β-cyclodextrin.%采用毛细管电泳-安培检测法测定了对羟基苯丙酮酸烯醇-酮式互变异构现象.采用以直径为0.3mm的碳微电极为工作电极的三电极系统,分离电压18kV,毛细管长度60cm,电泳液是添加了3mmol/L的β-环糊精溶液的pH=6.60、50mmol/L的磷酸盐缓冲液,两异构体在15min内实现基线分离.