血管活性肠肽对裸鼠移植瘤CD80和CD86表达的影响

摘要

目的 研究血管活性肠肽(VIP)对裸鼠移植瘤CD80、CD86表达的影响.方法 建立裸鼠皮下实体瘤模型,当接种了MKN45实体瘤的裸鼠的肿瘤体积约为30mm3时随机分为VIP组、VIP受体拮抗剂组、对照组3组,每组6只.皮下注射VIP或VIP受体拮抗剂10 μg· 100 ml-1·d-1,对照组PBS 100 μl/d.4周后处死裸鼠,取出移植瘤,用免疫组织化学方法检测移植瘤组织中CD80、CD86蛋白表达的变化.结果 CD80、CD86在VIP处理组、VIP受体拮抗剂处理组、对照组瘤块组织中均有表达.VIP组CD80的表达明显低于VIP受体拮抗剂组(P＜0.05)；VIP组CD86蛋白的表达显明低于对照组(P＜0.05)；而其他各组间两者的表达差异均无统计学意义(P＞0.05).结论 血管活性肠肽抑制裸鼠移植瘤中CD80、CD86的表达.%Objective To study the effect of vasoactive intesfinal peptide (VIP) on CD80 and CD86 expression in MKN45 tumor-burdened nude mice. Methods Experiment was started when MKN45 transplantation tumors had grown to approximately 30 mm in volume. Animals were randomly divided into 3 groups for treatment with VIP (VIP group, n=6, 10 μg · 100 ml · d ) , VIP antagonist ( antagonist group, n - 6, 10 μg · 100 ml · d~ ) and vehicle solution ( controls, n = 6, 100 μl/d ). Both compounds were injected subcutaneously. Tumor volumes and mice weights were measured weekly. Tumor weights were recorded at autopsy on the 28 day. To detect the expression of proteins of CD80 and CD86 in transplantation tumor tissues by immunohistrochemistry. Results The expression of protein of CD80 in transplantation tumor tissue of VIP group was lower than antagonist group (P < 0. 05 ). The expression of protein of CD86 in transplantation tumor tissue of VIP group was lower than control group (P < 0. 05). There was no significance between other two groups. Conclusion VIP inhibited the expressions of CD80 and CD86 in MKN45 transplantation tumor burdened nude mice.