Objective: U251 cells were exposed to fast neutrons of 14 MeV energy, and dose-response relationships were derived; Methods; cell samples were exposed to fast neutrons. Radiation doses were 1 Gy, 3 Gy, 5 Gy and 7 Gy respectively. The cell survival, apoptotic rate and proliferation were tested after irradiation; Result: with the growth of the radiation dose, cell survival was declined, and apoptotic rate was promoted; the speed of the cell proliferation, which was observed 48 h after irradiation, was slowed down obviously; Conclusion: compared to 晊 ray, fast neutrons have higher relative biological effectiveness.%目的:以14 MeV快中子照射体外培养的U251(人脑胶质瘤)细胞,研究快中子诱发U251的相关生物效应;方法:快中子照射U251细胞,吸收剂量分别为1、3、5、7 Gy,辐照后检测细胞的存活率、凋亡率、生长曲线以及周期变化等相关指标;结果:随着吸收剂量的增加,细胞存活率下降,凋亡率上升,呈良好的剂量-效应关系.照射后24h,细胞生长曲线无明显变化;照射后48 h,细胞增殖速度明显变缓;结论:快中子为高LET射线,与相关文献报道的γ射线相比,快中子具有较高的相对生物效应.
展开▼
