基于多波长透射光谱法的水体细菌微生物检测能力初步研究

摘要

搭建的水体细菌微生物多波长透射光谱快速测量实验系统,实验获取了肺炎克雷伯菌、金黄色葡萄球菌和大肠杆菌在不同浓度下220~900 nm范围内的多波长透射光谱,研究建立了三种细菌基于不同波长点及全光谱波段的浓度校准曲线,计算了肺炎克雷伯菌、金黄色葡萄球菌和大肠杆菌的检测限,并与紫外-可见分光光度计测量分析结果进行了对比.结果表明,实验系统与紫外-可见分光光度计测量光谱线性相关系数在0.9998以上,具有非常好的一致性,且30次光谱信号采集时间仅需15 s;基于实验系统分析得到三种细菌在220,258,300,350,400,450,500和550 nm不同波长点以及全光谱波段的检测限结果均优于紫外-可见分光光度计,且利用多波长透射光谱全光谱波段计算得到的细菌检测限均最低,其中:肺炎克雷伯菌、金黄色葡萄球菌和大肠杆菌的检测限分别为1.60×104,1.06×104和1.16×104 cells·m L-1.研究结果为进一步发展水体细菌微生物的多波长透射光谱快速定量检测技术提供了基础数据.%Based on a system for rapid measurement of multi-wavelength transmission spectra of bacteria ,multi-wavelength transmission spectra of Klebsiella pneumoniae ,Staphylococcus aureus and Escherichia coli in different concentrations of 220 to 900nm were obtained .The calibration curves of three kinds of bacteria based on the different wavelengths and the whole spectral band were established and the detection limits of Klebsiella pneumoniae ,Staphylococcus aureus and Escherichia coli were calculated .The results were compared with those measured by ultraviolet-visible spectrophotometer .The results showed that the linear correlation coefficient between the experimental system and the ultraviolet-visible spectrophotometer was above 0.9998 .In addition ,single measurement time of the average transmission spectrum measured by the system was 15 s ,which was 30 times of the signal acquisition .By analysis based on the experimental system ,the detection limits of three kinds of bacteria at different wavelengths of 220 ,300 ,350 ,400 ,500 ,550 nm and full spectrum were all better than those of ultraviolet-visible spectrophotometer .And the detection limit calculated by the full spectrum of the multi-wavelength transmission spectra was the lowest . The detection limit of Klebsiella pneumoniae ,Staphylococcus aureus and Escherichia coli were 1.60 × 104 ,1.06 × 104 and 1.16 × 104 cells · mL -1 .The results provide the basic data for the further development of the rapid and quantitative detection technology of the multi-wavelength transmission spectra of bacteria in water .