梅花鹿GHR基因完整编码区序列生物信息学分析

摘要

为了研究梅花鹿生长激素受体基因的结构和功能,从GenBank中下载梅花鹿、牛、山羊、猪、北极狐、大熊猫、人、猕猴、小鼠、大鼠、鸡、家鹅、绿头野鸭及金鱼的生长激素受体基因完整编码区及氨基酸序列,对梅花鹿与其他13个物种生长激素受体基因的完整编码区及其编码氨基酸序列进行相似性比对,并基于氨基酸序列构建系统进化树,利用BioEdit 7.0等软件对梅花鹿生长激素受体基因的碱基组成及其编码蛋白的理化性质和结构特征进行生物信息学分析.结果表明,梅花鹿与山羊、牛的生长激素受体基因氨基酸序列相似性较高,亲缘关系最近;梅花鹿生长激素受体基因完整编码区长度为1 905bp,编码634个氨基酸,A+T含量高于G+C;其编码的蛋白是一种分子质量为70.927 8ku、等电点为4.56的疏水性不稳定酸性蛋白;该蛋白含有1个信号肽,属于一种分泌型蛋白;存在1个强跨膜区、36个广泛磷酸化位点,二级结构元件以无规则卷曲为主.研究结果可为梅花鹿生长激素受体基因的进一步分析提供详细的生物信息学基础资料.%In order to study the structure and function of GHR gene of sika deer, coding sequences(CDS)of GHR gene in sika deer, cattle, goat, pig,arctic fox,giant panda,human,macaque, house mouse,norway rat,chicken,goose,mallard and goldfish were downloaded from GenBank as experimental materials. The similarity of GHR gene CDS and amino acid sequence between sika deer and other 13 species were analyzed. Phylo-genetic tree of the homologous gene based on the amino acid of GHR gene was constructed. In addition, we made bioinformatic analysis on basic information and encoding protein structure and physic - chemical property were predicted by means of biological software and online took.The results showed that the similarity comparison and phylogeneb'c tree indicated that the evolution distance of sika deer GHR gene was the most homogeneous to goat and cattle.The length of GHR gene coding sequence in sika deer is 1 905 bp, which encodes 634 amino acids.The estimated molecular weight of GHR protein is 70.927 8 ku.with an isoelectric point of 4.56 and 52.79 in instability index, belonging to the unstable acid protein with hydrophobieity. The GHR protein contained one signal peptide was probably being secreting type.The GHR protein had 1 obvious strong transmembrane region,36 phosphorylation sites.The secondary structure of GHR protein was mainly random coil.The research provides detailed bioinformatic informations for further study on GHR gene of sika deer.