HPLC法测定参松养心胶囊中人参皂苷Rb1的含量

摘要

建立参松养心胶囊中人参皂苷Rb1含量测定的HPLC方法.色谱柱Alltima C18(250mm×4.6mm,5μm),流动相乙腈-0.02％磷酸水溶液(32:68),流速1.0mL/min;检测波长203nm.人参皂苷Rb1进样量在0.4 ～ 4.0 μg与峰面积呈良好线性关系(r=0.999 1)；平均加样回收率100.3％,RSD 1.72％.本方法结果准确,精密度及重现性较好,可以用于参松养心胶囊中人参皂苷Rb1的含量测定.%To develop an HPLC method for determination of active ginsenoside Rb1 in Shensongyangxin Capsules.Chromatographic separation was performed on an Alltima C18 (250mm × 4.6mm,5μm)column; mobile phase was acetonitrile-0.02 ％ phosphoric acid,flow rate was 1.0mL/min; detector wavelength was 203nm.Good linear relationships of ginsenoside Rb1 was provided over investigated concentration between 0.4-4.0μg(r =0.999 1).The average recoveries of ginsenoside Rb1 was 100.3％.Repeatability experiments showed that relative standard deviation(RSD) values of ginsenoside Rb1 was 1.72 ％.The developed method was acurate,sensitive and repeatable,and can be used for the determination of ginsenoside Rb1 in Shensongyangxin Capsules.