麦穗鱼鳍条组织培养及染色体Ag-NORs和C-带研究

摘要

采用组织块贴壁培养法探索麦穗鱼(Pseudorasbora parva)鳍组织细胞的短期培养,同时对其染色体Ag-NORs和C-带进行了初步研究.结果显示:1)麦穗鱼鳍组织培养的最适培养基为MEM培养基,细胞在组织块贴壁后第6天迁出,经传代后细胞形态呈成纤维状；2)其染色体具有1对Ag-NORs,位于第23对染色体短臂末端,其NORs具多态性,未见Ag-NORs联合现象；3)其染色体C-带主要为端粒和着丝点C-带,少数为居间C-带,其中第23对染色体的短臂大部分呈现C-带强阳性,与Ag-NORs区域对应.%We investigated short-term fin tissues cultivation of Pseudorasbora parva by explant technique,and studied its Ag-NORs,and C-banding patterns at the same time.Results are as follows:1) Cells from the fin tissues of P.parva migrate out around tissues at 6th day through MEM medium,the most suitable one,and are fibroblast-like during cultivation; 2) A pair of Ag-NORs is found on the end of No.23 chromosome short arm,and the NORs are polymorphic,while no Ag-NORs combination has happened in this fish;3) C-bands are located mainly in the terminal and centromere regions of chromosomes,and a few in the interstitial regions.In particular,most of the short arm on chromosome No.23 shows strong positive C-bands,which is corresponded with Ag-NORs region.