油葵与光果甘草间作对根际土壤酶活性及微生物功能多样性的影响

摘要

通过盆栽试验，研究了新葵10号油葵与光果甘草间作对盐碱地根际土壤酶活性和微生物群落功能多样性的影响，以期为盐碱地的改良提供依据。结果表明，与单作模式相比，间作显著提高了根际土壤蔗糖酶、脲酶、磷酸酶和蛋白酶的活性。在144 h的温育期内，间作模式下的 AWCD均高于单作模式，并显著提高了 AWCD的利用率(72 h，P<0.05)。与单作模式相比，间作显著提高了微生物多样性指数(S、H)。主成分分析表明，间作优化了盐碱土壤微生物群落组成；羧酸类化合物、聚合物、氨基酸和碳水化合物是间作模式下根际土壤微生物利用的主要碳源。因此，新葵10号与光果甘草间作显著提高盐碱地根际土壤酶活性和微生物多样性指数，改变了微生物群落功能多样性，对盐碱土壤质量的改良有积极作用。%In order to provide a scientific basis for amelioration of saline-alkali soils, the pot experiment was conducted to study the effects of intercropping ofHelianthus annuus(Xinkui 10) andGlycyrrhiza glabra on enzyme activities and microbial diversity of saline-alkali rhizosphere soil. The results showed that, compared with the monoculture, intercropping of Xinkui 10 withGlycyrrhiza glabra significantly increased the activities of rhizosphere soil invertase, urease, phosphatase and protease. In 144 h incubation period, the AWCD of the intercropping treatment was higher than that of monoculture. The intercropping also significantly improved the utilization of AWCD(72h,P<0.05). Compared with monoculture, the intercropping significantly increased the microbial diversity indexes (S,H). Principal component analysis showed that the intercropping of Xinkui 10 with Glycyrrhiza glabra could optimize the microbial community composition of saline-alkali soils. The carbon sources mostly used by saline-alkali soil microbes were carboxylic acids, polymers, amino acids and carbohydrates. Therefore, the intercropping of Xinkui10 withGlycyrrhiza glabra has a positive effect on soil saline-alkali quality, because it significantly enhanced the related enzyme activities and soil microbial utilization degrees of carbon source, and changed the microbial diversities.