葡萄球菌肠毒素A联合PML-RARα多肽刺激特异性T细胞杀伤NB4细胞株的作用(英文)

摘要

Objective:To investigate the effects of staphylococcal enterotoxin A (SEA) on the cytotoxicity of T cells stimulated by PML-RARα peptide in vitro. Methods: Peripheral blood mononuclear cells (MNC) from healthy donor were obtained by density gradient centrifugation on Ficoll-Hypaque,MNC were cultured with PML-RARα peptide and SEA for 20 days. After induction,the cytotoxicity of T cells induced against NB4 and K562 cell lines were examined by Cell Counting Kit-8 (CCK-8). The CD4 and CD8 surface markers on the harvested CD3+ T cells were detected by flow cytometry (FCM). Results: The cyto-toxicity of T cells induced by PML-RARα peptide with SEA was higher than that of T cells induced only by PML-RARα peptide against NB4 cells. The FCM assay showed that the ratio of CD4+/CD8+ T cells were gradually decreased in both groups of PML-RARα peptide whether with SEA or not at the intervals of day 5,10 and 20 day after induction,but the most significantly decreased by PML-RARα peptide with SEA. Conclusion: The specific cytotoxicity of T cells induced by PML-RARα peptide against NB4 cells could be enhanced with superantigen SEA.