Inhibition of Telomerase Activity of Lymphoblastic Leukemic Cells by hTERT Antisense

摘要

To investigate the effect of antisense, human telomerase reverse transcriptase (hTERT) mRNA oligodeoxynucleotide on telomerase activity of lymphoblastic leukemic cells. Methods: Telomerase activity was measured by the telomerase PCR ELISA assay kit (TRAP), hTERT protein by immunochemistry and flowcytometry, hTERT mRNA expression by reverse transcription polymerase chain reaction (RT-PCR) assay and gel-image system. Results: Incubation of lymphoblastic leukemic cells (Jurkat, Raji and CEM cell lines) with 10 μmol/L AS PS-ODN could significantly decline the mRNA and hTERT after 72 h, and the telomerase activity was significantly down-regulated or inhibited. Conclusion: The hTERT AS PS-ODN was an excellent inhibitor for telomerase activity of lymphoblastic leukemic cells.