经颅磁刺激对局灶性脑缺血大鼠脑神经细胞凋亡、NO的影响

摘要

目的 观察经颅磁刺激对局灶性脑缺血大鼠脑组织的神经细胞凋亡数、NO的影响.方法 制备一侧大脑中动脉闭塞(MCAO)模型,造成大鼠左脑缺血24h,以改良的神经系统损害严重程度评分表(modified neurological severity score,NSS)评定神经功能缺损程度并筛选大鼠.随机分为对照组和磁刺激组(TMS).两组大鼠神经功能缺损无统计学差异.对照组给予5d假TMS刺激,TMS组给予5dTMS刺激.5d后采用干重湿重法测定脑含水量,采用流式细胞仪及硝酸还原酶法分别检测脑组织神经细胞凋亡率及一氧化氮含量变化.结果 TMS组大鼠的脑水肿程度明显减轻(P＜0.05)；TMS组神经细胞凋亡率及一氧化氮含量明显减少(P＜0.05).结论 TMS对脑缺血损伤具有神经保护作用.%Objective To observe effects of transcranial magnetic stimulation (TMS) on apoptosis rate and NO content after cerebral infarction in rats. Methods The rats are introduced to middle cerebral artery occlusion for 24hour. the extent of NSSwere evaluated. The rats were randomly divided into model group and TMS group, there is no statisticly difference of the NSS beteen the two groups. 5day' s TMS are applied to the TMS group. Results The severity of cerebral edema in TMS group was lessened (P< 0.05) , Apoptosis rate and NO content of TMS group are significantly lower than the model group(P<0. 05). Conclusion TMS may have a neuroprotective effect against focal cerebral injuries induced by ischemia.