异氟烷麻醉下肾切除术对老年大鼠学习记忆能力的影响

摘要

目的：探讨异氟烷麻醉下肾切除术对老年大鼠学习和记忆的影响。方法48只SD健康老年雄性大鼠随机平分为对照组和异氟烷麻醉下肾切除术组(麻醉手术组)。麻醉手术组大鼠在2%异氟烷(载气为含40%氧气的空氧混合气体)麻醉下行左侧肾切除术,麻醉时间为3h；对照组大鼠单纯吸入含40%氧气的空氧混合气体3h。实验干预后连续一周每日测定大鼠(每组12只)学习记忆能力,并在实验干预后1、7d,在未测定学习记忆能力的大鼠中随机每组选取6只大鼠测定血浆中细胞因子IL-6的浓度。结果术后第3d和第5d,麻醉手术组Y迷宫正确反应次数小于对照组,差异有统计学意义(P<0．05)。从术后第4d起,麻醉手术组Y迷宫主动反应次数明显小于对照组。术后第1、7d,麻醉手术组血浆IL-6浓度显著高于对照组,差异有统计学意义(P<0．05)。结论本研究成功建立异氟烷全身麻醉下肾切除术老年大鼠模型,并发现术后老年大鼠学习记忆能力下降。术后大鼠学习记忆能力降低可能与手术应激导致的IL-6血浆浓度升高有关。%Objective To investigate the affect of unilateral nephrectomy under isoflurane anesthesia on learning and memory in aged rats. Methods 48 healthy aged male rats were randomly assigned into two groups:40% oxygen inhalation for three hours only and left nephrectomy under 3-hour 2% isoflurane anesthesia. After treatment, learning and memory ability was assessed daily by using Y maze for one week(n=12). Serum level of interleukin-6 (IL-6) was determined at 1 and 7 days after treatment( n=6/subgroup) . Results At the 3 and 5 day after treatment, number of Y maze correct reactions ofrats enduring sur-gery under anesthesia was significantly less than thoseof rats in control group. From 4 day after treatment, number of Y maze active responses of rats enduring surgery under anesthesia was significantly less than those of rats in control group. At 1 and 7 day after treatment, serum level of IL-6 was significantly increased in rats of surgery under anesthesia group. Conclusion In our study, we successfully established anaged rat model of unilateral nephrectomy under isoflurane anesthesia. In those rats, the declined learn-ing and memory abilityand increased serum level of IL-6 werefound after treatment. These results suggested the increased serum level of IL-6 caused by surgical and anesthesia stress might contribute to learning and memory ability dysfunction.