香菇多糖对卵蛋白诱导小鼠急性哮喘模型免疫调节的影响

摘要

Objective To analyze the effect of lentinan on immune function of ovalbumin(OVA) induced mice acute asthma model the mouse model. Methods The BALBc female mice were randomly divided into 3 groups,control group (n=10), OVA group (n=10) and lentinan group (n=10). The OVA induced mice acute asthma model was built and lentinan was intrap-eritoneally injected 10mg/Kg,once a day,in all 14 days as intervention factor. Serum IgE,IgG level and IL-4,IFN-γconcentration in splenocyte supernatant were detected by ELISA,and Th1 expression level in splenocyte was detected by flow cytometry related antibody incubation and flow cytometer. Results IgE ofOVA group was significantly higher than that of lentinan group and control group,the difference being statistically significant (P<0. 05) and there being no statistically significant difference between lentin-an group and control group (P>0. 05). IgG of OVA group was significantly higher than that of lentinan group and blank control group,the difference being statistically significant ( P<0. 05 ) and no statistically significant differences between lentinan group and blank control group (P>0. 05). After using OVA to stimulate mononuclear cells of each group,cell viability was detected by MTS. Results showed that mononuclear cell viability of lentinan group decreased. Th1 cell proportion was detected by flow cy-tometer and results showed that Th1 cell proportion of OVA group was lower than control group and Th1 cell proportion of lentinan group was higher than OVA group. Compared with control group,IFN-γof OVA group significantly decreased,the difference being statistically significant (P<0. 05). IFN-γlevel of lentinan group was significantly higher than that of OVA group,the difference being statistically significant (P<0. 05),but no difference when compared with control group. Compared with the control group, IL-4 level of OVA group significantly increased,the difference being statistically significant (P<0. 05). IL-4 level of lentinan group was significantly higher than that of OVA group,the difference being statistically significant (P<0. 05),but there being no difference when compared with control group. Conclusion Lentinan may decrease OVA specific IgE by enhancing the synthesis of IFN-γ and inhibiting IL-4 synthesis,to achieve the goal of remitting and treating asthma.%目的：分析香菇多糖对卵蛋白( OVA)诱导小鼠急性哮喘模型免疫功能的影响。方法将balbc雌性小鼠,随机分为3组,空白组(n=10)、OVA组(n=10)、香菇多糖组(n=10)。建立OVA诱导小鼠急性哮喘模型,使用香菇多糖10mg/kg,1d/次,腹腔注射,共14d作为施加因素。以ELISA法检测血清中IgE、IgG水平及脾细胞上清中IL-4、IFN-γ浓度,用流式相关抗体孵育、流式细胞仪检测脾细胞Th1表达水平。结果 OVA组IgE显著高于香茹多糖组及空白组,差异有统计学意义(P<0．05),香茹多糖组与空白组比较差异无统计学意义(P>0．05)。 OVA组IgG显著高于香茹多糖组及空白组,差异有统计学意义(P<0．05),香茹多糖组与空白组比较差异无统计学意义(P>0．05)。用OVA刺激各组单个核细胞,MTS检测细胞活性,结果显示香菇多糖组单个核细胞活性降低,用流式检测仪检测Th1细胞比例,结果显示OVA组Th1比例低于正常组,香菇多糖组Th1比例高于OVA组。与空白组相比,OVA组IFN-γ水平显著降低,差异有统计学意义(P<0．05)；香茹多糖IFN-γ水平显著高于OVA组,差异有统计学意义(P<0．05),且与空白组无差异。与空白组相比,OVA组IL-4水平显著升高,差异有统计学意义( P<0．05)；香茹多糖IL-4水平显著高于OVA组,差异有统计学意义(P<0．05),且与空白组无差异。结论香茹多糖可能通过增强IFN-γ的合成,抑制IL-4的合成,降低OVA特异性IgE的水平,达到对哮喘的缓解和治疗的目的。