目的:研究昆明鼠经链佐霉素诱导后,血糖升高与学习和记忆能力之间的相关性.方法:选取年龄、体重以及血糖接近的昆明小鼠,连续5d腹腔注射50 mg·kg-1链佐霉素建立糖尿病小鼠模型.经血糖仪测定尾静脉血糖含量,其中空腹血糖大于或等于11 mmol· L-1作为糖尿病模型合格小鼠.糖尿病小鼠(n=12)和正常小鼠(n=10)通过水迷宫实验和穿梭实验检测小鼠学习和记忆能力.病理组织学检测糖尿病小鼠肾脏、胰腺和主动脉等器官病理改变.结果:与对照组相比,链佐霉素处理组小鼠饮食、饮水量显著提高并伴随体重下降(P＜0.05).4周后,糖尿病小鼠和正常小鼠的学习和记忆能力并没有显著差别(P＞0.05).但是12周后,糖尿病小鼠行动迟缓,比正常组小鼠定位巡航和空间探索能力均下降(P＜0.05).讨论:持续12周的高血糖状态并未导致STZ诱导的糖尿病小鼠出现认知功能障碍,有关糖尿病和认知功能之间的关系需要进一步进行研究.%Objective:To zdetermine the effects of hyperglycemia on learning and memory abilities in streptozotocin (STZ)-induced diabetic mice.Methods:Diabetes mellitus (DM) was induced in age-matched Kunming mice by STZ injection (50 mg · kg-1 body weight,intraperitoneally,once a day for 5 days).Diabetic status was assessed by blood glucose monitor.A fasting blood glucose level of more than or equal to 11 mmol · L-1 were considered as diabetic mice.The learning and memory abilities of the mice (12 for the diabetic mice and 10 for control) were evaluated by Morris water maze and the Shuttle box.Diabetic related pathological alternations were observed in kidney,pancreases,and aortaventralis.Results:Compared with sham group,STZ treatment significantly increased food consumption and water consumption as well as body weight loss (P＜0.05).DM mice did not show difference on learning and memory abilities compared with the control mice at the end of 4 weeks (P＞0.05),but they were mild sluggish and showed reduced navigation ability in the transfer test and the hidden platform test at end of 12 weeks (P＜0.05).Conclusion:Hyperglycemia itself did not induce cognitive dysfunction in STZ-induced diabetic mice.Further study should be carried out to clear the relationship between diabetes and cognitive disorder.
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