佩连麻黄方调节单纯性肥胖大鼠脂代谢紊乱的机制研究

摘要

Objective To observe the effect of Peilian mahuang prescription on lipid metabolism in simple o-besity rats,and to explore the possible anti-obesity mechanism of it.Methods A rat model of simple obesity was established with high fat diet and they were randomly divided into control group,model group,low,medium and high dose Peilian mahuang prescription groups.Peilian mahuang prescription was administrated intragastrically to obese rats for 8 weeks.Weight,body length and Lee's of rats were calculated.We peeled off white adipose tissue of epididymis and liver tissue.The volume of fat cell and number of lipid drops were detected by HE staining.The activity of AMPKαand the expression of PPAR-γ,SREBP-1c were detected by Real-time PCR and Western blot-ting.Results Peilian mahuang prescription could alleviate rat weight,reduce Lee′s level,activate AMPKα,re-strain the expression of PPAR-γand SREBP-1c,decrease lipogenesis and deposition.Conclusion Peptic Ulcer; Adiposity; Lipid metabdism disorders;Rats%目的 观察佩连麻黄方对单纯性肥胖大鼠脂代谢的影响,初步探讨其减肥机制.方法 采用高脂饮食喂养大鼠制备单纯性肥胖模型,随机分为对照组、模型组、低剂量佩连麻黄方治疗组、中剂量佩连麻黄方治疗组和高剂量佩连麻黄方治疗组.对治疗组进行相应灌胃给药,8周后测量大鼠体质量、体长,计算Lee指数;剥离附睾白色脂肪组织和肝脏组织,苏木精-伊红(HE)染色观察脂肪细胞体积和脂滴数量变化;结合实时定量聚合酶链反应、蛋白质印迹法(Western blotting)检测脂肪组织中腺苷酸活化蛋白激酶(AMPK)α信号活性和脂代谢相关指标过氧化物酶增殖物激活受体(PPAR)-γ、固醇调节元件结合蛋白-1c(SREBP-1c)的表达.结果 佩连麻黄方能够减轻单纯性肥胖大鼠体重,降低Lee指数,激活AMPKα,抑制PPAR-γ、SREBP-1c表达,减少脂肪生成和脂滴沉积.结论 佩连麻黄方对单纯性肥胖大鼠的减肥作用机制很可能是通过激活AMPKα来完成的.