P16蛋白在人脑胶质瘤中的表达

摘要

To observe the expression of P16 protein in human gliomas，theanti-P16 polyclonal antibody was applied to detect the expression of P16 protein in glioma cells by immunohistochemical method.The results showed that P16 pr otein was detected in plastic region of tumor cell.The frequency of positive exp ression of P16 protein in astrocytoge nic gliomas and ependymogenic gliomas wa s 42.9％ and 100％ respectively.The differ ence was significance（P＜0.025）in statist ic.The distribution of positive expressi on rate of P16 protein was different in different grade of gliomas.The differ ences were significant（P＜0.05）.This sugg ested that as the malignant degree o f gl ioma was raising， the expression rate of P16 protein in gliomas was decreasing. The deletions of P16 protein expressi on，e.g.the P16 gene homozygous deleti ons play an important role in malignant transformation of gliomas. The clinical results could be pre dicted by different expression levels of P16%采用免疫组化LSAB法检测P16蛋白在人脑胶质瘤中表达。结果显示：P16蛋白染色强度的分布在各级胶质瘤间存在显著性差异（P＜0.025），星形细胞起源的胶质瘤与室管膜细胞起源的胶质瘤P16蛋白阳性率分别为42.9％和100％，二者存在显著性差异（P＜0.05）；临床分期Ⅰ、Ⅲ期间亦存在显著差异。认为P16蛋白表达随胶质瘤恶性程度升高而降低，检测P16蛋白表达有助于判断胶质瘤的预后，并指导其治疗方案的选择。