Objective To observe the aberrant methylation of E-cadherin gene promoter in gastric carcinoma. Methods Aberrant methylation of E-cadherin gene promoter was examined by methylation specific-PCR (MSP) in gastric carcinoma and normal gastric tissues (n =54). Results Aberrant methylation rate of E-cadherin gene promoter was 48. 1% (26/54) in gastric carcinoma tissues and and 11. 11 % (6/54) in normal gastric tissues respectively (P < 0.05). There were significant correlation between E-cadherin gene promoter methylation rate and pathology type, clinical staging, depth of tumour invasion and lymphatic metastasis. Conclusion Methylation of E-cadherin is a common molecular event and may be related to invasion and metastasis of gastric carcinoma.%目的 观察胃癌组织中E-cadherin基因启动子异常甲基化状态.方法 采用甲基化特异性聚合酶链反应(MSP)技术检测54例胃癌及其癌旁正常组织中的E-cadherin基因启动子异常甲基化状态,并分析E-cadherin基因启动子异常甲基化状态与胃癌临床病理参数的关系.结果 胃癌组织中的E-cadherin基因启动子异常甲基化发生频率为48.10％ (26/54),明显高于癌旁正常组织中的11.11％ (6/54).E-cadherin基因启动子异常甲基化频率与胃癌分化程度、病理类型、浸润深度以及淋巴结转移、临床分期有关(P均＜0.05).结论 胃癌组织中E-cadherin基因启动子异常甲基化频率较高,并与胃癌浸润、转移有关.
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