IFN-λ对人食管癌细胞增殖的抑制作用及机制

摘要

目的 探讨干扰素lambda(IFN-λ)对6种人食管癌细胞株生长增殖的影响并分析其作用机制.方法 采用RT-PCR法检测6种食管癌细胞株IFN-λ受体表达.采用IFN-λ食管癌细胞株干预食管癌细胞株,MTT法检测细胞增殖抑制率:胎盘蓝染色后计数活细胞数:流式细胞术分析各细胞周期分布.结果 6种人食管癌细胞均表达IFN-λ受体:IFN-λ对各食管癌细胞生长均有不同程度的抑制作用:在TE11,YES5、T.Tn细胞株中,IFN-λ作用后72 h可见TE11、YES5、T.Tn活细胞数明显减少:TE11细胞的G1期升高:YES5、T.Tn细胞的Sub-G1期明显升高.结论 IFN-λ体外可抑制食管癌细胞增殖,其机制为诱导细胞凋亡并将细胞阻滞于G1期.%Objective To investigate the growth inhibition of IFN-lambda( IFN-λ) on 6 human esophageal carcinoma cells and analyze the mechanism. Methods The expression of IFN-λ receptors was examined with RT-PCR in 6 human e-sophageal carcinoma cells. The esophageal carcinoma cells were treated with IFN-λ, then the cell proliferation was evaluated with MTT assay. The kinetics of cell growth was examined with the trypan blue dye exclusion test. The distributions of cell cycle were analyzed with flow cytometer. Results Six esophageal carcinoma cells all expressed IFN-λ receptors. IFN-λ mediated the growth inhibition with the differential sensitivity. In TE11, YES5 and T. Tn cells, viable cell numbers after IFN-λ treatment reduced significantly. IFN-λ enhanced the G, phase in TE11 cells and sub-G1 phase in YES5 and T. Tn cells. Conclusion IFN-λ can inhibit the growth of esophageal carcinoma cells in vitro through G1 phase arrest or apop-tosis.