汉族癫痫患者ABCB1基因C3435T多态性与血清、脑脊液P-gp表达的关系

摘要

Objective To investigate the relationship between polymorphism at C3435T of ABCB1 and serum and cerebrospinal fluid (CSF) P-gp expression in Han people with epilepsy. Methods 118 cases of Han people with epilelsy were collected in the study, which included 57 cases of antiepileptic drugs (AEDs) sensitive patients and 61 cases of AEDs resistant patients. CC genotype in epilepsy patients was 39, and non-CC genotype patients was 79. ELISA method was used to detect the P-gp in serum and cerebrospinal fluid, and ABCBl_C3435T genotypes were detected by polymerase chain reaction _restriction fragment length polymorphism (PCR-RFLP) method. Results There was no statistical difference in Pgp in serum or CSF or the ratio of serum P-gp and CSF P-gp between epilepsy patients with non-CC genotype and patients with CC genotype, CC genotype or non-CC genotype AEDs sensitive and resistant patients ( all P＞0.05). Conclusion There is no relationship between ABCBl_C3435T polymorphism and P-gp expression and AEDs tolerance in Han people with epilepsy.%目的 探讨汉族癫痫(EP)患者ATP结合盒蛋白B亚家族成员1(ABCB1)基因C3435T多态性与脑脊液、血清P-gp表达的关系.方法 选择汉族EP患者118例,其中对抗癫痫药物(AEDs)敏感57例、耐药61例;ABCB1基因CC型39例、非CC型79例.采用ELISA法检测患者的脑脊液及血清P-gp,PCR-RFLP分析ABCB1 C3435T基因型.结果 CC型、非CC型,CC型或非CC型对AEDs敏感及耐药患者的脑脊液、血清P-gp及其比值比较,均无统计学差异(P均＞0.05).结论 ABCB1基因C3435T多态性与EP患者的P-gp表达、AEDs耐药均无相关性.