Objective To investigate the preparation of chitosan encapsulated poms calcium polyphosphate ( CPP) bioceramic and its combination with osteoblasts in vitro, in order to find a kind of biomaterial which can be used in the treatment of bone defect. Methods The chitosan microsphere was produced by method of latex coagulation and was used as the porogen. The porous CPP bioceramic was produced by method of microspheres accumulation and it was encapsulated by the chitosan by infiltration method. After that, the mechanical property of the chitosan encapsulated porous CPP bioceramic was tested. The rabbits bone marrow mesenchymal stem cells were isolated and induced into osteoblasts. We combined the CPP compound with the osteoblasts. The osteoblast morphologic and functional detection were performed. Results The CPP bioceramic turned out to be good at perforation and uniform holes inside. The compressive strength of CPP was 400 Mpa. The osteoblasts showed good appearance, cellularity and function after their combination with the chitosan encapsulated porous CPP bioceramic in vitro. Conclusions The chitosan encapsulated porous CPP bioceramic is good in both appearance and mechanical properties and it showed good compatibility with rabbit osteoblasts.%目的 探讨壳聚糖包裹的多孔聚磷酸钙(CPP)生物陶瓷的制备方法及其与兔成骨细胞在体外复合的情况,以探索一种可以应用于骨缺损治疗的生物材料.方法 采用乳液凝固法合成壳聚糖微球并将其作为后续步骤的致孔剂.采用微球堆积法制备多孔CPP,并通过浸润法使壳聚糖膜包裹于其上,随后对其进行生物力学性能测试.分离培养兔骨髓间充质干细胞并诱导成为成骨细胞,将其与壳聚糖膜包裹的多孔CPP生物陶瓷复合,观察复合后的成骨细胞生长情况,检测其生理活性.结果 制备出的多孔CPP生物陶瓷孔的分布较为均匀,贯通性较好,抗压强度约为400 MPa.成骨细胞与壳聚糖膜包裹的多孔CPP生物陶瓷复后成骨细胞形态、生长良好,并且可以分泌出细胞外基质.结论 壳聚糖膜包裹的多孔CPP生物陶瓷结构及力学性能良好,在体外与成骨细胞相容性良好,为骨组织工程支架材料的研究提供了新的思路.
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