催产素对大鼠创伤后应激障碍的预防作用

摘要

目的 探讨催产素对大鼠创伤后应激障碍( PTSD)的影响. 方法 将Wistar大鼠24只随机分为空白对照组、单纯应激组、催产素组各8只. 单纯应激组、催产素组给予大鼠单次延长应激( SPS)刺激,其中催产素组在应激后12 h内给予催产素干预,共干预14 d. 干预过程中每隔3 d评价各组拒缚反射评分;干预第16天各组给予旷场实验,观察其穿格行为和站立行为;第17~21天行Morris水迷宫实验,观察其定位航行能力、空间探索能力. 结果 与单纯应激组比较,催产素组拒缚反射评分降低,旷场实验穿格次数和站立次数增多,定位航行能力及空间探索能力改善,两组比较差异均有统计学意义(P均<0.05). 结论 应激后早期及时给予催产素干预可明显减轻大鼠的应激相关表现,并且对应激造成大鼠的学习记忆能力损伤有保护作用.%Objective To investigate the effect of oxytocin on post-traumatic stress disorder ( PTSD) of rats.Meth-ods Twenty-four Wistar rats were randomly divided into the control group, the single prolonged stress ( SPS) group and the oxytocin ( OT) group with 8 rats each.The 14-day oxytocin intervention was given to rats after the SPS stimulation with-in 12 hours.During the intervention, the scores of bound refusing reflection were evaluated every three days.On the 16th day, the open-field test was conducted in each group to observe the crossing and the standing behaviors;on the 17th to 21st days, the Morris water maze test was done to observe the navigation capability and the space exploration ability.Results Compared with the SPS group, the scores of bound refusing reflection in the OT group decreased, the times of crossing and standing behaviors increased and the navigation capability and the space exploration ability improved, thus the differences in these two groups were significant ( all P<0.05) .Conclusions An early and timely oxytocin intervention can signifi-cantly reduce the stress-related clinical manifestations in rats and protect the injury on learning and memory ability of rats caused by stress.