HBx 基因转染对人肝癌细胞系 HepG2细胞增殖的影响

摘要

Objective To investigate effect of HBx transfection on HepG 2 cell proliferation .Methods The HBV X gene eukaryon expression vector pcDNA 3-X was transiently transfected into HepG 2 cell by lipid-media transfection .Untras-fected HepG2 and HepG2 transfected with pcDNA3 were used as control .Growth status of liver cancer cells was observed by microscopic , proliferation of liver cancer cells in different group was detected by cell-flow and fluorescence microscopy , respectively.Results The growth of liver cancer cells in transfected X gene group was better than that of the other two groups.The HepG2 cells in the transfected HBx group were in the stage of proliferation ( G2 was 5.68%).The rate of pro-liferation of transfected HepG 2 cells was 47%, the rate of proliferation of HepG 2 transfected with pcDNA 3 or untrasfected HepG2 cells was 28%, 25%, respectively.The rate of proliferation of transfected HepG2 cells was higher than that in oth-er two groups(all P<0.05).Conclusions HBx gene transfection can result in proliferation of hepatoma carcinoma cells .%目的：探讨HBx基因转染对人肝癌细胞系HepG2细胞增殖的影响。方法用脂质体转染法将HBx真核表达载体pcDNA3／HBx瞬时转入HepG2细胞（转染HBx细胞组）；以转染空载体细胞组和未转染HepG2细胞组作为对照。以流式细胞仪及荧光显微镜观察各组肝癌细胞增殖情况。结果转染HBx细胞组的HepG2肝癌细胞生长较其他两组密集。流式细胞仪检测显示转染HBx细胞组的HepG2细胞较多处于增殖期（ G2期细胞占5．68％）。荧光显微镜图像显示转染HBx细胞组处于增殖期的HepG2细胞比率为47％，转染空载体细胞组为28％，未转染HepG2细胞组为25％；转染HBx细胞组HepG2细胞处于增殖期的比率与其他两组比较有统计学差异（ P均＜0．05）。结论 HBx基因转染能促进人肝癌细胞系HepG2细胞增殖。