非吸烟女性肺腺癌患者肿瘤组织 EGFR 基因突变、miR-25表达与生理生育特征的关系

摘要

Objective To investigate the correlations between epidermal growth factor receptor ( EGFR) mutation, ex-pression of miR-25 and female physiology and fertility factors in female patients with non-smoking lung adenocarcinoma. Methods We collected the female physiology and fertility factors ( age, menarche age, menopausal status, age of meno-pause, the number of live births and menstrual period) in 130 female patients with non-smoking lung adenocarcinoma.We detected EGFR mutation by using DNA sequencing technology and the expression of miR-25 by real-time quantitative PCR. The correlations between EGFR mutation, the expression of miR-25 and female physiology and fertility information were detec-ted by chi-square test, Logistics regression and Wilcoxon rank test, respectively.Results There were 71 patients with EG-FR mutation, and 59 cases with wild type EGFR in 130 female patients.EGFR mutation was not related with age, menopaus-al status, age of menopause, the number of live births and menstrual period (all P>0.05), but was related with female men-arche age (P=0.041).The adjusted Logistics regression analysis showed that the patients with menarche age more than 14 years old were not more likely to EGFR mutation than those of menarche age less than 14 years old (OR=0.376, 95%CI=0.146-0.970).The miR-25 expression was not related with age, female physiology and fertility factors of patients.Conclu-sions EGFR mutation is associated with female menarche age in female patients with non-smoking lung adenocarcinoma. However, there is no correlation between the expression of miR-25 and female physiology and fertility factors.%目的：分析非吸烟女性肺腺癌患者肿瘤组织EGFR基因突变、miR-25表达与生理生育特征的关系。方法130例非吸烟女性肺腺癌患者，收集患者的生理生育信息（年龄、初潮年龄、绝经状态、绝经年龄、活产次数、行经时间），采用DNA测序技术检测患者肿瘤组织EGFR基因，采用实时荧光定量PCR法检测miR-25表达，采用χ2检验及Logistics回归分析 EGFR基因突变与女性生理生育特征的关系，采用Wilcoxon秩和检验分析肿瘤组织miR-25表达与女性生理生育特征关系。结果130例非吸烟女性肺腺癌患者中71例EGFR基因出现突变，59例仍为野生型EGFR。 EGFR基因突变与非吸烟女性肺腺癌患者年龄、绝经状态、绝经年龄、活产次数、行经时间无关（P均＞0．05），与初潮年龄有关（P＝0．041）；经多因素调整Logistics回归分析显示，初潮年龄≥14岁的非吸烟女性肺腺癌患者较初潮年龄＜14岁者更不易反生EGFR基因突变（OR＝0．376，95％CI：0．146～0．970）。肿瘤组织中miR-25的表达与患者年龄及女性生理生育因素均无关。结论非吸烟女性肺腺癌患者肿瘤组织EGFR基因突变与初潮年龄有关；miR-25表达与上述女性生理生育因素均无关。