目的 观察舌鳞状细胞癌(TSCC)组织中Oct4、Sox2蛋白表达变化,并探讨其临床意义.方法 采用免疫组化SP法检测83例TSCC组织、15例舌正常黏膜组织中Oct4、Sox2蛋白表达,分析Oct4、Sox2蛋白阳性表达率与TSCC患者临床病理参数的关系.随访5年,记录患者生存率,采用COX比例分析Oct4、Sox2蛋白阳性表达率与5年生存率的关系.结果 TSCC组织中Oct4、Sox2蛋白阳性表达率高于舌正常黏膜组织(P均<0.05).Oct4、Sox2蛋白阳性表达率均与患者年龄、性别、吸烟史、饮酒史、肿瘤临床分期无关(P均>0.05),与肿瘤组织学分级、淋巴结转移有关(P均<0.05).Oct4或Sox2蛋白阳性表达的患者5年生存率较表达阴性者低(P均<0.05),Oct4和Sox2蛋白同时阳性表达的患者总生存率最低(P均<0.05).Sox2及淋巴结转移是TSCC患者生存的独立影响因素(P均<0.05).结论 TSCC组织中Oct4、Sox2蛋白呈高表达,二者可能参与TSCC的发生发展,且Sox2是患者生存的影响因素.%Objective To observe the expression changes of Oct4 and Sox2 proteins in tongue squamous-cell carcino-ma (TSCC)and to explore its clinical significance. Methods The expression of Oct4 and Sox2 proteins in 83 TSCC tis-sues and 15 normal mucosa tissues was detected by immunohistochemical SP. The relationship between the expression of Oct4 and Sox2 proteins and the clinicopathological parameters of TSCC patients was analyzed. Patients were followed up for 5 years. The survival rate was recorded. The relationship between the positive rate of Oct4 and Sox2 protein expression and the 5-year survival rate was analyzed by COX ratio. Results The positive expression rates of Oct4 and Sox2 proteins in TSCC tissues were higher than those in the normal tongue tissues (P < 0. 05). The positive expression of Oct4 and Sox2 proteins was not related to age,sex,smoking history,drinking history,and tumor clinical staging (all P > 0. 05),but was related to the histological grade and lymph node metastasis (both P < 0. 05). The 5-year survival rate of Oct4 or Sox2 pro-tein positive patients was lower than that of those with negative expression (P < 0. 05). The total survival rate of patients with Oct4 and Sox2 positive protein expression was the lowest (P < 0. 05). Sox2 and lymph node metastasis were independ-ent factors affecting the survival of TSCC patients (all P < 0. 05). Conclusion The expression of Oct4 and Sox2 protein is highly expressed in TSCC,both of them may participate in the development of TSCC,and Sox2 is the influencing factor for the survival.
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