实时定量 PCR 检测食品中单核细胞增生性李斯特氏菌

摘要

单增李斯特氏菌广泛存在于肉类、蛋类、禽类、海产品、乳制品、蔬菜等食品中，其中毒严重的可引起血液和脑组织感染。本研究通过实时荧光定量 PCR 及常规方法对2份单增李斯特氏菌进行能力验证，旨在检验我院检测能力，进而提升我院的综合实力。荧光定量PCR 检测单增李斯特氏菌较常规方法更加灵敏、简便和快速！%Listeria monocytogenes was widely found in meat,eggs,poultry,seafood,dairy products,vegetables and other foods, poisoning can cause serious blood and brain infections. 2 Listeria monocytogenes were detected by real - time PCR in the CNAS proficiency testing in this study. To test our detection capability,and upgrade our comprehensive national strength. Real - time PCR for detection of Listeria monocytogene compared with the conventional methods is more sensitive,simple and fast!