The inbred line QR-001 with high resistance to maize rough dwarf virus ( MRDV) as female parent , and the highly susceptible inbred line QS -001 as male parent , were used to produce a F2 segregation mapping population including 281 individuals .1 028 pairs of SSR primers , which distributed evenly though -out the genome in maize ( Zea mays L.) , were used for screening polymorphisms between the parental lines . And 110 polymorphic SSR markers were identified .The linkage group covering 10 chromosomes were construc-ted using the software QTL IciMapping Version 3.3.The total length of linkage group was 1 343.2 cM, and the even distance of each marker interval was 10 .3 cM.Only 1 molecular marker ( bnlg161 ) was not linked with the population .There were 26 .6%of all the SSR markers occurring segregation distortion .The linkage group was helpful for the MRDV QTL mapping , molecular marker-assisted selection and molecular design of resist-ance breeding .%以自育的高抗玉米粗缩病自交系QR-001为母本、高感自交系QS-001为父本，配制了包含281个单株的F2分离群体为作图群体。选用在玉米基因组中均匀分布的1028对SSR引物在双亲间进行多态性引物的筛选，共检测到110个可以鉴别F2群体各单株标记基因型的SSR多态性标记。借助QTL IciMapping Version 3．3软件构建了较好覆盖玉米10条染色体的分子标记连锁图谱，图谱总长度为1343．2 cM，标记间平均距离为10．3 cM。其中1个分子标记（bnlg161）不与连锁群连锁。所建连锁群中有26．6％的SSR分子标记发生偏分离。该图谱的构建为玉米抗粗缩病QTL定位、分子标记辅助选择和分子设计抗病育种等研究奠定了基础。
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