库尔勒香梨组织培养的研究

摘要

Korla fragrant pear has soft flesh and delicious flavor,which is one of the important agricul-tural exports of China.While,there are problems of low fruit setting rate and unstable yield of Korla fragrant pear.Improving the productivity traits of fruit tree by genetic transformation based on tissue culture is a signifi-cant method for high efficiency breeding.In this paper,the in vitro tissue culture method of Korla fragrant pear was established using tender buds as explants.The effects of disinfection time of 0.1% HgCl2 solution on sterilizing effect and browning of explants were studied and the optimum multiplication medium and rooting me-dium were explored.The results showed that during the tissue culture process of Korla fragrant pear,the opti-mum disinfection time of 0.1% HgCl2 was 5 minutes;the optimum multiplication medium was MS +1.5 mg/L 6 -BA +0.1 mg/L IBA +1.5 mg/L GA3;and the optimum rooting medium was 1 /2MS +0.05 g/L AC +1.0 mg/L IBA.%香梨果实肉质细腻，酸甜可口，是我国重要的出口创汇农产品之一。目前，香梨普遍存在坐果率低、产量不稳定的问题，通过组织培养进行遗传转化改良性状是当前果树高效育种的重要策略。本试验以库尔勒香梨茎段萌发的嫩芽为材料，通过离体组织培养，研究0．1％HgCl2溶液消毒时间对外植体灭菌效果和褐化的影响，并探讨了适宜的增殖培养基和生根培养基配方。结果表明，库尔勒香梨组织培养过程中，0．1％HgCl2消毒的适宜时间为5 min；适宜的增殖培养基为 MS ＋1．5 mg／L 6－BA ＋0．1 mg／L IBA ＋1．5 mg／L GA3；适宜的生根培养基为1／2MS ＋0．05 g／L AC ＋1．0 mg／L IBA。