水稻α－NAC 基因家族的克隆及生物信息学分析

摘要

Nascent polypeptide -associated complex (NAC)is a heterodimer consisting of αand βsub-units.There are three members of α-NAC in rice genome,which are localized in chromosome 1,3 and 5 re-spectively.The full open reading frames of the three α-NAC genes were cloned by RT -PCR from Oryza sati-va cv.Zhonghua 11.Further bioinformatics analysis revealed that the amino acid sequence of Osα-NAC was conserved in NAC and UBA domains,and showed the highest similarity to ZmNACs.Os1gNAC shared 67.4% and 81.8% homology with Os3gNAC and Os5gNAC respectively,whereas Os3gNAC and Os5gNAC shared 69.5% homology.These results would provide references for protein purification and structural biologi-cal study,and lay a foundation for functional analysis of Osα-NAC.%新生多肽结合复合体（Nascent polypeptide －associated complex，NAC）是由α和β两个亚基组成的异二聚体复合物。水稻新生多肽结合复合体α链基因家族共有3个基因，分别位于1、3号和5号染色体上。本研究根据公布的水稻基因组序列，通过 RT －PCR 方法克隆了粳稻品种中花11号中α－NAC 家族的3个基因。经生物信息学分析发现，水稻α－NAC 基因家族的氨基酸序列比较保守，包含 NAC 和 UBA 两个结构域，与玉米 NAC 基因家族的亲缘关系最近。Os1gNAC 和 Os3gNAC、Os5gNAC 的相似性分别为67．4％、81．8％， Os3gNAC 和 Os5gNAC 的相似性为69．5％。本研究结果为 Osα－NAC 蛋白纯化及蛋白结构生物学研究提供参考，为今后 Osα－NAC 基因家族的生物学功能研究奠定基础。