萝卜离体再生体系的建立

摘要

To select the best hormone combination for inducing adventitious shoots and adventitious roots,the in vitro culture of cotyledons with petiole from 8 genotypes of radish were carried out in this research.The results showed that Xinuoxiaoqing and Cuilüradish had high -frenquency regeneration potential.The optimum medium for their adventitious shoot induction was MS +30 g·L -1 Sucrose +0.7% Agar +5 mg·L -1 6 -BA +0.05 mg·L -1 NAA,and that for adventitious root induction was MS +30 g·L -1 Sucrose +0.7% Agar +5 mg·L -1 6 -BA +0.10 mg·L -1 NAA.%以8种不同基因型的萝卜为试验材料进行带柄子叶离体培养，筛选诱导不定芽和不定根的最佳培养基配方。结果表明：喜诺晓青和翠绿萝卜具有高频再生潜力，由其带柄子叶诱导不定芽的最佳培养基为：MS 培养基＋30 g·L －1蔗糖＋0．7％琼脂＋5 mg·L －16－BA ＋0．05 mg·L －1 NAA，诱导不定根的最佳培养基为：MS 培养基＋30 g·L －1蔗糖＋0．7％琼脂＋5 mg·L －16－BA ＋0．10 mg·L －1 NAA。