苹果植株应答连作障碍差异蛋白质组学分析

摘要

为明确不同苹果品种应对连作障碍时差异表达的蛋白,在蛋白表达水平上揭示苹果应答连作障碍的机制,本研究选择在重茬地中表现不同的两个苹果品种(抗重茬能力强的富士2001和抗重茬能力弱的首富1号)作为试材,利用重茬土进行盆栽试验,运用蛋白质非标记定量技术(label-free)鉴定两个苹果品种叶片中差异表达的蛋白,将差异蛋白与GO、COG等数据库进行比对,分析不同苹果品种差异蛋白的功能和分类.结果表明,两个苹果品种在重茬土栽植条件下主要鉴定得到43种差异或特异表达的蛋白,主要分为4类,包括光合作用相关蛋白(1种)、植物能量及物质代谢相关蛋白(17种)、植物防御和抗逆性相关蛋白(8种)、其他功能蛋白(17种),其中,植物能量及物质代谢、植物防御和抗逆性相关蛋白表达量具有较大差异.不同苹果品种在连作障碍反应相关蛋白的表达量方面存在差异,抗重茬能力强的品种蛋白表达量明显高于抗重茬能力弱的品种,推测参与防御反应的苹果过敏原蛋白和病程相关蛋白是苹果植株应对连作障碍的关键蛋白.抗重茬能力强的品种通过加强多种不同途径的代谢,增强植株对于连作障碍的抗逆性,减少连作障碍对植物的伤害.%This research aims to explore the mechanism of apple in response to continuous cropping obstacle at the protein expression level by confirming the differential expression proteins of different apple cultivars answering continuous cropping.Stronger replanting-tolerant cultivar Fuji 2001 and weaker replantingtolerant cultivar Shoufu 1 were grown in pots with replanted soils.Under the same cultivation and management conditions,label-free technology was used to identify the differential expression proteins in the leaves of apple which had different resistance abilities to continuous cropping.The differential expression proteins were compared with GO,COG and other databases to analyze their function and classification.The results showed that 43 kinds of differential or particular expression proteins were identified from two apple cultivars,and were mainly divided into four categories,including one protein related to photosynthesis,seventeen proteins referring to energy and carbohydrate metabolism,eight proteins related to plant defense and resistance,and seventeen proteins with other functions.There were great differences among the proteins of energy and carbohydrate metabolism and plant defense and resistance.Different apple cultivars had different protein expression levels related to continuous cropping.Furthermore,the stronger replanting-tolerant apple cultivar had higher protein expression level than the weaker one.It was inferred that the allergen proteins and pathogenesis related proteins,which joined in defensive reaction,were the key proteins of apple in response to continuous cropping.The stronger replanting-tolerant apple cultivar enhanced various different metabolic pathways to strengthen the resistance to continuous cropping obstacle and reduce the harm to plants.