黄芪总皂苷对脾虚大鼠胃黏膜损伤的抑制作用和血流量的改善作用研究

摘要

目的:探讨黄芪总皂苷对脾虚大鼠胃黏膜损伤的抑制作用和黏膜血流量的改善作用研究.方法:随机将60只SPF级SD雄性大鼠分为5组:正常组、模型组和黄芪总皂苷小、中、高剂量组.除正常组之外,其余各组建立大鼠脾虚模型,待造模4周后,小、中、高剂量组给予黄芪总皂苷灌胃,剂量分别为25、50、100 mg/kg,正常组、模型组给予相同体积的生理盐水灌胃.各组持续灌胃2周.观察各组大鼠的指标.结果:与正常组对比,模型组的黏膜血流量、肠道灌流液IgA和血清NO、SOD、IgG均明显的降低,血清MDA升高(P<0.01);与模型组对比,黄芪总皂苷各剂量组的黏膜血流量、肠道灌流液IgA和血清NO、SOD、IgG均升高,黏膜损伤指数和血清MDA降低(P<0.05或 P<0.01).结论:黄芪总皂苷能抑制脾虚大鼠黏膜损伤,改善黏膜血流量,这可能与提高机体免疫功能和抑制脂质过氧化作用有关.%Objective:To explore inhibitory effect of gastric mucosal injury and improving role of gastric mu-cosal blood flow in spleen deficient rat by total saponins of Astragalus.Methods:60 SPF SD male rats were random-ly divided into normal group,model group,and total saponins of Astragalus of small,medium and high dose group. In addition to normal group,the rest were established model of spleen deficient,after 4 weeks,total saponins of As-tragalus of small,medium and high dose group was given total saponins of Astragalus,with dose of 25,50 and 100 mg/kg,respectively,normal group and model group was given same volume of physiological saline lavage.Each group was continued to fill stomach for 2 weeks.Each index of rats were observed.Results:Compared with normal group,model group of gastric mucosal blood flow,IgA intestinal perfusion fluid and serum NO,SOD,IgG were significantly reduced,serum MDA increased(P<0.01);Compared with model group,gastric mucosal blood flow, IgA intestinal perfusion fluid and serum NO,SOD and IgG of total saponins of Astragalus each dose group rise,gas-tric mucosal damage index and serum MDA lower(P<0.05 or P<0.01).Conclusion:Total saponins of Astragalus can inhibit rat gastric mucosal injury,and improve gastric mucosal blood flow,this may be related with improving body's immune function and inhibition of lipid peroxidation.