补肾强骨汤治疗糖尿病性骨质疏松大鼠模型疗效研究

摘要

目的:观察补肾强骨汤治疗糖尿病性骨质疏松(DO P)大鼠的疗效.方法:60只实验大鼠,随机选取10只纳入空白组,其余50只大鼠制备为2型糖尿病性DO P大鼠模型,选取建模成功的30只大鼠随机均分为模型组、阳性对照组与观察组;模型组不予任何治疗干预,阳性对照组以安慰剂治疗,观察组给予自拟补肾强骨汤治疗,12周后观察各组大鼠血糖、胰岛素、血钙、血磷、碱性磷酸酶、骨密度(BMD)以及血清胰岛素样生长因子(IGF-1)、肿瘤坏死因子-α(TNF-α)的变化情况.结果:模型组、阳性对照组、观察组大鼠空腹血糖、空腹胰岛素与空白组均具有差异(P<0.05);模型组与阳性对照组无差异(P>0.05);观察组空腹血糖低于模型组、阳性对照组,空腹胰岛素高于模型组、阳性对照组,差异具有统计学意义(P<0.05).模型组、阳性对照组、观察组大鼠血钙、血磷、碱性磷酸酶及BM D与空白组比较,差异具有统计学意义(P<0.05);模型组与阳性对照组无差异(P>0.05);观察组血钙、血磷、碱性磷酸酶及BM D均优于模型组与阳性对照组(P<0.05).模型组、阳性对照组、观察组大鼠血清IGF-1、TNF-α表达水平与空白组比较,差异具有统计学意义(P<0.05);模型组与阳性对照组无差异(P>0.05);观察组IGF-1、T N F-α表达水平均优于模型组与阳性对照组(P<0.05).结论:自拟补肾强骨汤具有降低糖尿病性DO P大鼠血糖水平,改善骨质疏松增加骨密度的明确疗效.%Objective:To observe the curative effect of Bushen Qianggu decoction on diabetic osteoporosis(DOP)rats. Methods:The purchase of 60 rats,10 rats were randomly selected into the blank group,the remaining 50 rats were prepared for DOP of type 2 diabetic rats,30 rats were selected randomly for modeling the success of model group and positive control group and the observation group;model group was not given any treatment intervention,positive control in the placebo group,the observation group was treated with Bushen Qianggu decoction in the treatment of rats were observed,blood glucose,insulin,serum calcium, phosphorus,alkaline phosphatase,bone density after 12 weeks(BMD)and insulin-like growth factor(IGF-1),tumor necrosis fac-tor alpha(TNF-α)changes.Results:The model group,positive control group,the observation group of rats with fasting blood glucose,fasting insulin and control group have differences(P<0.05);no difference between model group and positive control group (P>0.05);fasting glucose in observation group lower than the model group,positive control group,fasting insulin was higher than that of model group,positive control group,had significant difference(P<0.05).Model group,positive control group,the obser-vation group of rats serum calcium,phosphorus and alkaline phosphatase and BMD and the blank group have differences(P<0. 05);no difference between model group and positive control group(P>0.05);observation group,serum calcium,phosphorus and alkaline phosphatase and BMD were better than that of model group and positive control group(P<0.05).Model group,positive control group,the observation group rats serum IGF-1,TNF-expression level and the blank group have differences(P<0.05);no difference between model group and positive control group(P>0.05);observation group IGF-1,TNF-expression level was better than the model group and positive control group(P<0.05).Conclusion:The Bushen Qianggu decoction can reduce the blood sugar level of diabetic DOP rats,improve the osteoporosis and increase the bone mineral density.