外源ZnCl2对成县迟蒜盐胁迫响应研究

摘要

In different concentrations of ZnCl2 on Chengxian late garlic bulb seeds under salt stress,the seed germination,seedling growth and the contents of osmotic adjustment substances and 4 kinds of antioxidant enzyme activities were determined.The results show that under the single NaCl stress,garlic bulb seed germination and seedling growth were severely suppressed,after adding different concentrations of ZnCl2,the germination rate of garlic bulbs and the salt tolerance of seedlings were significantly increased.When the concentration is 10-5 mol/L,garlic seed germination rate,germination index and shoot length,root length,root number,and seedling fresh weight,seedling dry weight,root fresh weight,root dry weight and root volume,SOD,POD,CAT and APX antioxidant enzymes activity reached the maximum,the content of soluble sugar,proline and MDA decreased to the lowest level.Therefore,exogenous ZnCl2 could alleviate the optimal concentration of NaCl stress on garlic bulb seed germination and seedling injury,the optimum concentration was 10-5 mol/L.%用不同浓度的ZnCl2对盐胁迫下成县迟蒜鳞茎种子进行处理,对大蒜鳞茎种子萌发、幼苗生长和幼苗中渗透调节物质的含量及4种抗氧化酶活性进行了测定.结果表明,在单一NaCl胁迫下,大蒜鳞茎种子萌发和幼苗的生长受到严重抑制,添加不同浓度ZnCl2后,大蒜鳞茎种子萌发率和幼苗的耐盐性明显增强.添加浓度为10-5 mol/L时,大蒜鳞茎种子萌发率、萌发指数和芽长、根长、发根数以及幼苗鲜重、苗干重、根鲜重、根干重和根体积、抗氧化酶SOD、POD、CAT和APX活性达到最大,可溶性糖、脯氨酸和丙二醛含量降到最低.因此,外源ZnCl2缓解NaCl胁迫对大蒜鳞茎种子萌发和幼苗伤害的最适浓度为10-5 mol/L.