纳米材料钼对大豆子叶节诱导的影响

摘要

为提高大豆子叶节转化效率,以豫豆22为试验材料,研究不同浓度(0,20,40,60 mol/L)纳米材料钼(Mo)对大豆子叶节的影响.结果表明,纳米材料钼对出芽、抽茎和生根都有促进作用,在0～40 mol/L范围内随着浓度的升高逐渐增加,40 mol/L时达到最大值,出芽率、抽茎率和生根率分别比对照提高了13.54％、13.18％和11.96％;在60 mol/L时,3项指标均下降,出芽率仍显著高于对照,抽茎率降至与对照无显著差异,生根受到抑制,生根率下降了4.76％,显著低于对照.因此,在0～40 mol/L浓度范围内,向培养基中添加纳米材料钼可以提高大豆子叶节的诱导效果.%To improve the transformation efficiency of soybean cotyledonary node,different concentration nano materials of molybdenum(Mo) were conducted to study the effects of soybean cotyledonary node of Yudou 22.The results show that the nano materials of molybdenum have a promoting effect on budding,pumping stems and rooting which gradually increased with the increase of concentration during 0-40 mol/L,and the 40 mol/L of concentration reached the maximum,the budding rate,pumping stems rate and rooting rate improve by 13.54％,13.18％ and 11.96％ respectively.The three index have declined at 60 mol/L,budding rate was significantly higher than control,pumping stems rate dropped to no significant differences compared with control,root was restrained,rooting rate dropped by 4.76 ％ and significantly lower than control.Therefore,adding nano materials of molybdenum at 0-40 mol/L into the culture medium can improve the induction effect of soybean cotyledon node.