桑天牛羧酸酯酶和谷胱甘肽S-转移酶与杨树次生代谢物质相关性研究

摘要

Activities of carboxylesterase and glutathioneStransferase in %Apriona germari% larvae which feeding on eight different kinds of poplar trees were tested.The results showed that the activities of the two enzymes were significantly different when the larvae feeding on different poplar trees.The contents of fourteen secondary metabolites in eight poplar trees were also tested with the method of highpressure liquid chromatography (HPLC).By cluster analysis the eight poplar trees were divided into groups.One group includes %P.canadensis,P.×euraericana% cv.“I214”,%P.×beijingensis,%J2 poplar,J1 poplar and%P.canadensis% cv.“Shanhaiguanensis”.The other group includes%P.tomentosa, P. alba% var.%pyramidalis.%Stepwise regression analysis was carried out to test the relationship between the activities of the above enzymes and secondary metabolites of poplar trees.The analysis result demonstrated that the contents of phenol and phyadroxybenzoic acid decreased together with the increase of carboxylesterase activity while the contents of syringic acid and chlorogenic acid increased,but the content of ferulic acid decreased together with increasing of glutathioneStransferase activity while the content of phenol in poplar trees increased.%对取食8种杨树的桑天牛幼虫羧酸酯酶和谷胱甘肽S-转移酶活力进行了测定，证实取食不同杨树的桑天牛幼虫羧酸酯酶和谷胱甘肽S-转移酶活力存在显著差异。采用高效液相色谱法对8种杨树的14种次生代谢物质含量进行了测定，经聚类分析将8种杨树分为2类：加拿大杨、I-214杨、北京杨、J-2杨、J-1杨、山海关杨为一类，毛白杨、新疆杨为另一类。对羧酸酯酶和谷胱甘肽S-转移酶活力与杨树次生代谢物质进行了逐步回归分析，逐步回归方程表明，杨树丁香酸、绿原酸含量增高，苯酚、对羟基苯甲酸含量降低，则羧酸酯酶活力会较高；杨树苯酚含量增高，阿魏酸含量降低，则谷胱甘肽S-转移酶活力会较高。