Quadratic regressive factorial experiment was used to optimize concentrations of 6-BA and NAA added in MS midum for shoot multiplication of miniature rose ( Rosa cv. Sonnen Kind). The most amount of shoots was achieved by combining 6-BA 1.93 mg·L-1, NAA 0.33mg·L-1 into MS midum. The 6-BA and NAA concentrations were in a range of 1.68～2.75mg·L-1 and 0.07～0.54mg·L-1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%.
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