@@ 泡桐(Paulownia)是经济价值较高的优良绿化造林树种.由于丛枝病的危害,每年我国林业的直接经济损失就高达数千万元,并且严重影响农民种植泡桐的积极性(杨俊秀等,2007).%In this study, the trichosanthin (TCS) gene, which was cloned from Trichosanthes kirilowii, was transformed into Paulownia elongata through agrobacterium-mediated method. The conversion rate was 29.2％ with 86 transformed strains obtained. The target gene had approximately 50％ positive expression rate in plantlets through 11 times subculture and PCR detection for 4 times. And the PCR detection of the plants in field showed that the positive rate was between 40％ and 50％. No positive expression of the labeled product of witches' broom was found in the transformed plants after infection susceptible experiment and molecular detection, meanwhile, the virus infection rate of the control was up to 15.0％. Thus, it indicated that, compared to the control, the transformed plants showed higher resistance to the witches'broom disease.
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