大豆对大豆疫霉菌株Pm14抗性的遗传分析及基因定位

摘要

[目的]研究大豆对大豆疫霉菌株Pm14的遗传模式,并对其抗性基因进行定位.[方法]采用下胚轴创伤接种方法进行抗性鉴定,利用苏88-M21与新沂小黑豆构建的重组自交系群体进行遗传分析,并通过SSR技术结合BSA方法对苏88-M21的抗性基因进行定位.[结果]苏88-M21对大豆疫霉菌株Pm14的抗性由1对主效基因控制,抗病对感病表现为显性.通过分子作图,将抗性基因RpsSu定位于大豆分子遗传图谱0连锁群微卫星标记Satt358和Sat-242之间,与这2个标记的遗传距离分别为3.5 cM和7.4 cM.[结论]苏88-M21对大豆疫霉菌株Pm14的抗性是由1对单显性基因控制的,并将在苏88-M21发现的抗性基因RpsSu定位于0连锁群.%[Objective] The objective of this study was to reveal the inheritance pattern and map resistance gene to Phytophthora sojae (P. sojae) strain Pm14 in soybean. [Method] A population of 176 recombinant inbred lines (RIL) derived from the cross of Su88-M21 ×Xinyixiaoheidou was evaluated for phytophthora resistance using the hypocotyl inoculation technique. The RIL was used to analyze the inheritance of resistance and map the resistance gene to a linkage group. [Result] The results showed that the resistance of Su88-M21 to strain Pm14 is controlled by a single gene (Rps), and the resistance is dominant to susceptible.RpsSu (resistance gene in Su88-M21 was temporarily designated) is a novel phytophthora resistance gene which was firstly found on molecular linkage group O, and flanked by Satt358 and Sat_242 with genetic distances 3.5 cM and 7.4 cM. [Conclusion] The resistance to Pm14 is controlled by a single dominant gene (Rps), and the resistance gene RpsSu in Su88-M21 was firstly mapped on molecular linkage group O.