高效液相色谱法同时测定柑橘果实中18种类黄酮的含量

摘要

[目的]建立一种高效液相色谱法同时测定柑橘果实中18种类黄酮(圣草次苷、二氢槲皮素、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、野漆苷、槲皮苷、圣草素、香峰草苷、枸橘苷、柚皮素、木犀草素、山奈酚、香叶木素、橙黄酮、川陈皮素、橘皮素)的含量.[方法]色谱柱为ZORABX SB-C18(4.6 mm×250 mm,5μm),流动相为甲醇-甲酸-水,梯度洗脱,流速为0.7 mL·min-1,柱温25℃,检测波长黄烷酮为283 nm、黄酮和多甲氧基黄酮为330 nm、黄酮醇为367 nm.[结果]18种类黄酮在42 min内完全分离,线性范围为62.4-960 mg·L-1(r=0.9996-1.0000),定量限为0.05-0.133 μg·mL-1,回收率为88.70％-104.76％,相对0.33％-3.05％ (n=6).[结论]该方法简便、快速、准确、可靠,适合柑橘果实中18种类黄酮的含量测定.%[Objective] This study aimed to investigate a high-performance liquid chromatograph (HPLC) method for the determination of different kinds of flavonoids, namely, eriocitrin, taxifolin, narirutin, naringin, hesperidin, neohesperidin, rhoifolin, quercitrin, eridictyol, didymin, poncirin, naringenin, luteolin, kaempferol, diosmetin, sinensetin, nobiletin and tangeretin in citrus fruits. [Method] The chromatographic separation was performed on a ZORABX SB-C18 column kept at 25°C using methanol /formic acid/water mixture as the mobile phase at a flow rate of 0.7 mL-min-1 through gradient elution and the detection wavelength was set at 283 nm for flavanones, 330 nm for flavones and polymethoxylated fiavones and 367 nm for flavonol. [Result] Results showed that the thorough separation of 18 flavonoids was achieved within 42 min. The method exhibited a linear range of 62.4-960 mg-L-1 (r=0.9998-1.0000) and limits of quantification (LOQ) ranging from 0.05-0.133 ug-mL-1. The average recovery rates of this method were 88.70%-104.76%, with a relative standard derivation range of 0.33%-3.05%. [Conclusion] This method is simple, rapid, accurate , reliability, and can be used for the determination of flavonoids in citrus fruits.