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Identification of Kalidium species (Chenopodiaceae) by DNA barcoding

机译:DNA条形码识别钾盐物种(藜科)

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摘要

DNA barcoding is an increasingly prevalent molecular biological technology which uses a short and conserved DNA fragment to facilitate rapid and accurate species identification.Kalidium species are distributed in saline soil habitat throughout Southeast Europe and Northwest Asia,and used mainly as forage grass in China.The discrimination of Kalidium species was based only on morphology-based identification systems and limited to recognized species.Here,we tested four DNA candidate loci,one nuclear locus (ITS,internal transcribed spacer) and three plastid loci (rbcL,matK and ycflb),to select potential DNA barcodes for identifying different Kalidium species.Results showed that the best DNA barcode was ITS locus,which displayed the highest species discrimination rate (100%),followed by matK (33.3%),ycflb (16.7%),and rbcL (16.7%).Meanwhile,four loci clearly identified the variant species,Kalidium cuspidatum (Ung.-Sternb.) Grub.var.sinicum A.J.Li,as a single species in Kalidium.
机译:DNA条形码技术是一种日益流行的分子生物学技术,它利用一种短而保守的DNA片段来促进快速准确的物种鉴定。钾物种分布在整个东南欧和西北亚的盐渍土壤生境中,在中国主要用作草料。钾盐物种的区分仅基于基于形态学的识别系统,并且仅限于公认的物种。在这里,我们测试了四个DNA候选基因座,一个核基因座(ITS,内部转录间隔子)和三个质体基因座(rbcL,matK和ycflb),结果表明,最好的DNA条形码是ITS基因座,它显示出最高的物种识别率(100%),其次是matK(33.3%),ycflb(16.7%)和rbcL (16.7%)。同时,四个基因座清楚地鉴定出了变种,即虎杖(Kalidium cuspidatum)(Ung.-Sternb。)Grub.var.sinicum AJLi,是钾中的一个物种。

著录项

  • 来源
    《寒旱区科学(英文版)》 |2017年第1期|89-96|共8页
  • 作者

    XiaoHui Liang; YuXia Wu;

  • 作者单位

    State Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China;

    State Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
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  • 入库时间 2022-08-19 03:41:28
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